Microscale: reagents addition

The addition of reagents to a reaction can be carried out, in the simplest case, directly with the aid of a microspatula in the case of solids or with an automatic pipette, or a Pasteur pipette for liquids or solutions.

If the reaction is to remain in a closed vessel, the reagents should be added through septum plugs, punctured with a syringe fitted with a needle and the reagent added.

Sometimes, the puncture of a second needle is necessary to facilitate the process and avoid overpressure. In the Figure, the simplest case is described, where a reagent is added to a reaction tube.

When the setups are somewhat more complex, such as a reflux, septum type stoppers or similar are also used to add the reagents from a syringe. The septum should be placed directly over the vessel in which the reaction is heated so that the contents of the syringe drip directly into the flask or conical vial.

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